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Bovine tuberculosis is highly infectious zoonotic disease of economic and public health importance. This study aimed to detection the prevalence of bovine tuberculosis by bacteriological examination and Real time PCR. A total of 75 animals (61 cattle and 14 buffaloes) were routinely examined in the slaughterhouses of Menufia governorate, Egypt during the period of 2015 and 2016 for detection of tuberculosis. The suspected tuberculous lesions collected from cattle were from respiratory, from digestive, from head, mixed lesions and generalized lesions. While in buffaloes, the tuberculous lesions were from respiratory, from digestive, mixed lesions and from head lesions. The bacteriological examination revealed that the isolation rate of Mycobacterium bovis (M. bovis) was 86.9% and 57.1% in cattle and buffaloes, respectively. The real time PCR technique was applied to confirm the results of bacteriological examination and revealed that 90.2% and 85.7% M. bovis isolates in cattle and buffaloes respectively were positive with high sensitivity and specificity more than culture.

Key words

Bovine tuberculosis, Mycobacterium bovis, Menufia, PCR.