Studies on ruminant brucellosis in El Salam canal area, Egypt

I.M.Wassif1, R.H Mohamed2, and A. M.El-Kattan 1"


The current study is aimed to provide some epidemiological data about brucellosis and its impact among ruminants in the area around El-Salam canal, North Sinai. In addition to determination of the sensitivity, specificity; positive & negative predictive values and diagnostic efficacy of Brucella Antibody Test Kits as a rapid field assay for the diagnosis of brucellosis. Our results revealed that, the overall prevalence rate of brucellosis was 7.1% (12/168) and 10.1% (17/168) using ELISA and Brucella Antibody Test Kits respectively. Out of 168 serum samples; 9 were seropositive and 148 were sero-negative for brucellosis by both ELISA and Brucella Antibody Test Kits. Another 3 and 8 serum samples were only positive with ELISA and Brucella Antibody Test Kits respectively, revealing 75% sensitivity, 94.8% specificity, 47% positive predictive value, 53.5% negative predictive value and 93.4% diagnostic efficacy for this test. Brucellosis was found to be one of the causes of abortion in the study area as 61.9% (13/21) of aborted animals were seropositive for brucellosis. While to a little extent, it can be considered one of infertility causes as 4.7% (7/147) of infertile animals were seropositive for brucellosis. On the other hand, there were 38% (8/21) and 95.2% (140/147) of aborted and infertile animals respectively were sero-negative for brucellosis revealing that, there were another causes of abortion and infertility in the area of study rather than brucellosis and there was a necessity for their further investigations in order to improve the fertility and productivity of the animals in this targeted area.

Key words

Brucellosis, Ruminant, El Salam Canal, ELISA.