Improvement of Inactivated Rabies Vaccine using Montanide Pet Gel-A

S.M.Edries, A.S.Amani, A.M.Albehwar, and S.E.E.Hemmat"


The present work was designed to investigate the ability to use montanide pet gel-A (polymeric adjuvant manufactured under Good Manufacturing Practice, GMP rules) in the production of inactivated cell culture rabies vaccine for pet animals. The test of the National Institute of Health (NIH) indicated that antigenic values were 2.5, 2.0 and 3.0 for the reference, aluminum hydroxide gel and montainde pet gel-A vaccines, respectively. The prepared montanide pet gel-A vaccine was found to be safe and immunogenic for experimental cats and dogs. Smaller dose of the prepared vaccine (1ml/cat or dog) was found to be immunogenic for such animals instead of a dose of 2ml of the aluminum based vaccine. Serum Neutralization Test (SNT) and antigen specific Enzyme Linked Immunosorbent Assay (ELISA) were used to assess the immune response induced in vaccinated cats and dogs showing titers of 64, 64 and 128 by SNT and 5, 5 and 6 log2 by ELISA in dogs and 64, 64 and 128 by SNT and 5, 5 and 5.5 log2 by ELISA in cats vaccinated with the reference, aluminum hydroxide gel vaccine and pet gel A vaccine respectively. Montanide pet gel-A could be recommended to be used as an adjuvant to rabies vaccine for pet animals using smaller dose and inducing higher antibody levels. With smaller doses high production rate could be suggested.

Key words

Rabies vaccines, Pet gel-A, Aluminum hydroxide gel, Serum neutralization test, Enzyme linked immunosorbent assay.