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Chicken infectious anemia virus (CIAV) has been placed in the list of emerging viruses that cause severe threat to the Egypt poultry industry. Enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) were applied for testing CIAV in vaccinated chicks in two experiments. In experiment (1) chicks at 1-day old were subcutaneously vaccinated with original stock or diluted preparations (from 10-1 to 10-6) from commercial CIAV-vaccinal strain (Cux strain). In experiment (2) chicks at 7-day old were vaccinated with experimentally contaminated live NDV vaccine with CIAV Cux strain by eye drop (G2) parallel to that vaccinated with CIAV subcutaneously (G1) and other control non-vaccinated group (G3). ELISA detected CIAV antibodies in sera of vaccinated chicks with original stock or diluents preparations (till 10-3) from Cux strain at 21day post-vaccination (dpv) while CIAV DNA was detected in thymus, spleen and liver in most of the vaccinated chicks with original stock or diluents preparations (till 10-6) at 14 dpv by PCR. For experiment (2) ELISA could detect CIAV antibodies in sera by the second week post-vaccination in G1 and by the third week post-vaccination in G2 while PCR able to detect CAV DNA in liver and spleen tissues by the second week post-vaccination in G1 and G2. In conclusion, the commercial CIAV- Cux strain induce humoral response associated with viral persistency in spleen, thymus and liver in vaccinated chicks limit its usage in young chicks. PCR and ELISA were tests of choice for current testing CIAV in vaccinated chicks.

Key words

CIAV, ELISA , PCR, Vaccinated Chicks.