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Volatile oils obtained from Rosmarinus officinalis plants treated with recommended doses of organic and mineral fertilizers (VO1); plants treated with organic, mineral and bio fertilizers (VO2); plants treated with combination of organic, mineral, bio fertilizers and Biomagic (VO3) were analyzed by Gas chromatography (GC), it was illustrated that VO3 was better than VO1 and VO2 concerning known chemical constituents. Moreover, VO1, VO2 and VO3 were screened for their antibacterial, antioxidant, antitumor and antidiabetic activities. VO3 were more effective in inhibiting the growth of tested bacteria whereas VO1 was the least inhibitive. On the other hand the antitumor results revealed that VO3 exhibited the lowest inhibitory concentration 50 (IC 50) against MCF-7 carcinoma cell line in comparison to the other oils. The three oils were analyzed for their antioxidant activities using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH%) scavenging activity method. VO3 revealed the highest DPPH scavenging % in comparison to the other oils. The results showed that alpha glucosidase diabetes enzyme was inhibited by all the tested oils in dose dependent manner but with superiority to VO3. This study showed great prospects in effectiveness of biofertilizers in combination with Biomagic on constituents of R. officinalis oil and subsequently on its biological activities. Thus, this study points to investigate the combination of biofertilizers and Biomagic as a potential tool to improve biological activities of plant volatile oils.

Key words

Rosmarinus officinalis oils, GC, Biotechnology, Antibacterial, Antitumor, Antioxidant, Antidiabetic.